Gonadotropin Releasing Hormone (GnRH) Stimulation Test

Indication: To evaluate patients who present with low LH and sex steroids. The test may also be used to diagnose patients with delayed puberty.

Procedure:

1. Insert saline lock into peripheral vein
2. Obtain blood samples for baseline LH and FSH (red top tubes)
3. Inject 100ug GRH (IV) through saline lock
4. Obtain blood samples for LH and FSH at 15, 30, 45, 60 and 120 minutes
5. Inject 3cc 0.9% normal saline into saline lock after drawing blood samples to prevent blood from clotting
6. Waste 5cc blood prior to drawing blood samples

Interpretation: The interpretation of this test is dependent on the patient's gender, age, sex and reason for performing the test. Some patients may require priming with GnRH before the stimulation test is performed. The test results should be evaluated by an endocrinologist.